MPC-Polymer

A fully synthetic, water-soluble polymer with a unique monomer composition designed for the manufacturing of superior IVD and biochemical assay reagents.

MPC (2-Methacryloyloxyethyl Phosphoryl Choline) consists of phosphoryl choline groups and methacrylic groups. Phosphoryl choline group is hydrophilic imparting high biocompatibility and high hydrophilicity.

MPC based polymer materials are especially produced for diagnostic applications such as IVD additives and assay reagents. The rich variation in physical property derived from its monomer composition, renders MPC-Polymer to have unique features and advantages.

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Aziza Aknin
Key Account Manager

Applications

The unique featurs of MPC-Polymer make it superior to protein-based reagents.

Immunoassay

Western Blotting

Immunohisto-chemistry

Agglutination Assay

Lateral Flow Tests

Bead Based Assays

Features

MPC-Polymer are fully chemically synthesized substances, therefore exhibiting little biohazardous risk and little lot-to-lot variation.

Synthetic polymer  (MPC-Polymer)

Protein-based reagents

Lot-to-lot variation

no

yes

Danger of infection

no

yes

Stability

high

low

Reproducibility

high

high

Impurity

no

yes

MPC-Polymer effectively suppresses lot-to-lot variation.

Advantages

Blocking Effect

MPC-Polymer effectively suppresses non specific adsorption of protein on microplate or magnetic beads.

This experiment shows a 50 % better performance compared to the common material BSA.

Figure 1: A MaxiSorp (Thermo Fisher Scientific) 8-well plate was blocked with 10-fold diluted MPC-Polymer (0,5 wt% final, diluted with PBS).

Protein Stabilization Effect

MPC-Polymer effectively stabilizes proteins on solid or liquid phase materials.

MPC-Polymer and its hydrated water surrounds proteins to optimize the hydrophobic balance, and suppresses denaturation, aggregation, and non-specific adsorption to inside wall of vessel.

This experiment shows a 50 % better performance compared to the common material BSA.

Figure 2: A anti-mouse IgG antibody was immobilzed on a MaxiSorp (Thermo Fisher Scientific) 8-well plate and afterwards blocked with 10-fold diluted MPC-Polymer (0,1 wt%).

Sensitizing Effect (Latex Agglutination Test)

MPC-Polymer effectively improves the sensitivity of latex agglutination tests.

Figure 3: A latex agglutination test detecting CRP (C-reactive protein) was carried out to evaluate the performance of MPC-Polymer. The test was performed using 7070 automated analyzer (Hitachi). MPC-Polymer was added to the sample diluent and to the latex particle suspension (1 wt% final concentration).

Sensitizing Effect (Lateral Flow Test)

MPC-Polymer significantly improves the sensitivity of lateral flow tests.

Figure 4: A lateral flow test detecting SARS-CoV-2 IgM and IgG antibody was carried out using a combination of two MPC-Polymers in the final concentration 0,75 wt% in the buffer solution.

Product Line

We recommend to choose for the initial evaluation MPC-Polymer according to application and purpose (below table).

We offer a wide range of MPC-Polymers with various physical properties. In some cases a combination of two or more different MPC-Polymers are necessary. We are happy to find out your best MPC-Polymer (combination) to meet your needs.

MPC Polymer Series

Property

Application

Specification

MPC 100 Series

Very hydrophilic

(neutral)

Latex agglutination

Lateral flow test

Sensitizing

MPC 200 Series

Moderately hydrophobic

ELISA / CLEIA

Latex agglutination test

Lateral flow test

Blocking

MPC 400 Series

Very hydrophilic

(anionic)

Lateral flow test

Metal surface modification

Sensitizing

MPC 800 Series

Relatively hydrophobic

(BSA-like property)

ELISA / CLEIA

Lateral flow test

Blocking

Stabilizing

MPC 1000 Series

Moderately hydrophobic

ELISA / CLEIA

Blocking

MPC 1200 Series

Moderately hydrophobic

ELISA / CLEIA

Latex agglutination test

Blocking